6/5/2023 0 Comments G protein linked receptors![]() ![]() Y. Fang, A. G. Frutos, B. Webb, Y. Hong, A. Ferrie, F. Lai, J. Lahiri: Membrane biochips, BioTechniques Suppl, 62–65 (2002) Y. Fang, A. G. Frutos, J. Lahiri: Membrane protein microarrays, J. T. Mirzabekov, H. Kontos, M. Farzan, W. Marasco, J. Sodroski: Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5, Nat. K. L. Martinez, B. H. Meyer, R. Hovius, K. Lundstrom, H. Vogel: Ligand binding to G protein-coupled receptors in tethered cell membranes, Langmuir 19, 10925–10929 (2003)Ī. Waller, P. Simons, E. R. Prossnitz, B. S. Edwards, L. A. Sklar: High throughput screening of G-protein coupled receptors via flow cytometry, Comb. P. Banks, M. Harvey: Considerations for using fluorescence polarization in the screening of G protein-coupled receptors, J. 5, 297–306 (2000)Ĭ. J. Daly, J. C. McGrath: Fluorescent ligands, antibodies, and proteins for the study of receptors, Pharmacol. J. C. Owicki: Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer, J. 107, 133–149 (2004)ĭ. Fotiadis, Y. Liang, S. Filipek, D. A. Saperstein, A. Engel, K. Palczewski: The G protein-coupled receptor rhodopsin in the native membrane, FEBS Lett. N. C. Santos, M. A. Castanho: An overview of the biophysical applications of atomic force microscopy, Biophys. J. M. Edwardson, R. M. Henderson: Atomic force microscopy and drug discovery, Drug Discov. ![]() ![]() G. Tollin, Z. Salamon, V. J. Hruby: Techniques: plasmon-waveguide resonance (PWR) spectroscopy as a tool to study ligand-GPCR interactions, Trends Pharmacol. 3, 639–650 (2002)ĭ. K. Vassilatis, J. G. Hohmann, H. Zeng, F. Li, J. E. Ranchalis, M. T. Mortrud, A. Brown, S. S. Rodriguez, J. R. Weller, A. C. Wright, J. E. Bergmann, G. A. Gaitanaris: The G protein-coupled receptor repertoires of human and mouse, Proc. K. L. Pierce, R. T. Premont, R. J. Lefkowitz: Seven-transmembrane receptors, Nat. This process is experimental and the keywords may be updated as the learning algorithm improves.Ī. Wise, K. Gearing, S. Rees: Target validation of G-protein coupled receptors, Drug Discov. These keywords were added by machine and not by the authors. Green Fluorescent Protein Fusion Protein.The latter points should greatly extend the range of applications to which technologies based on GPCRs could be applied. This chapter focuses on cell-free GPCR assay nanotechnologies and describes some molecular biological approaches for the construction of more sophisticated, surface-immobilized, homogeneous, functional GPCR sensors. ![]() Stable, robust, cell-free signaling assemblies comprising receptor and appropriate molecular switching components will form the basis of future GPCR/G-protein platforms, which should be able to be adapted to such applications as microarrays and biosensors. With growing interest and commercial investment in GPCRs in areas such as drug targets, orphan receptors, high-throughput screening of drugs and biosensors, greater attention will focus on assay development to allow for miniaturization, ultrahigh-throughput and, eventually, microarray/biochip assay formats that will require nanotechnology-based approaches. Ligand recognition by the receptor leads to the activation of a generic molecular switch involving heterotrimeric G-proteins and guanine nucleotides. G-protein coupled receptors (GPCRs) underpins a multitude of physiological processes. ![]()
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